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sf21 expression systems 94 003 t ni  (Expression Systems Inc)


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    Expression Systems Inc sf21 expression systems 94 003 t ni
    Sf21 Expression Systems 94 003 T Ni, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 99/100, based on 4781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sf21+expression+systems+94+003+t+ni/Sf9+Cells/pm38401543-233-212-213
    Average 99 stars, based on 4781 article reviews
    sf21 expression systems 94 003 t ni - by Bioz Stars, 2026-10
    99/100 stars

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    Virus:

    Article Title: Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 8WG16 Millipore-Sigma Cat# 05-952-I; RRID: AB_492629 Bacterial and virus strains Escherichia coli DH5-alpha New England Biolabs C2987 Escherichia coli BL21 (DE3) RIL Merck 230280 Escherichia coli DH10EMBacY Geneva Biotech N/A Biological samples Porcine thymus tissue Pel-Freez Biologicals 59451-2 Chemicals, peptides, and recombinant proteins RNA Polymerase II Vos et al., 201840 N/A SPT4 (DSIF) Bernecky et al., 201762 N/A SPT5 (DSIF) Bernecky et al., 201762 N/A NELF-A (NELF) Vos et al., 201654 N/A NELF-B (NELF) Vos et al., 201654 N/A NELF-D (NELF) Vos et al., 201654 N/A NELF-E (NELF) Vos et al., 201654 N/A NELF-A residues 1-276 (NELF) This paper N/A NELF-A R252D, V254R, L257K, D258R (NELF) This paper N/A NELF-A K272D, R273E, R274E, R275E, K276D This paper N/A TFIIS Vos et al., 201840 N/A TFIIS (DE-AA) Vos et al., 201840 N/A Deposited data Pol II-DSIF-NELF paused, post-translocated (Map A) This paper PDB: 8UHD EMD-42270 Pol II-DSIF-NELF paused, tilted (Map B) This paper PDB: 8UHA EMD-42267 Pol II-DSIF-NELF paused, post-translocated (Map C) (map only) This paper EMD-42304 Pol II-DSIF-NELF poised, pre-translocated (Map D) This paper PDB: 8UI0 EMD-42285 Pol II-DSIF-NELF poised, post-translocated (Map D) This paper PDB: 8UBG EMD-42280 Pol II-DSIF-NELF-TFIIS, poised (Map E) This paper PDB: 8UIS EMD-42303 Experimental models: Cell lines Sf9 Expression Systems 94-001 Sf21 Expression Systems 94-003 T. ni (Hi5) Expression Systems 94-002 Oligonucleotides Template 5’- CCA CTG GAA GAT CTG AAT TTA CGG GCG CAA CTA TGC CGG ACG TAC TGA CC -3’ Sigma-Aldrich N/A Non-template 5’- GGT CAG TAC GTC CGG CAT AGT TGC GCC CGT AAA TTC AGA TCT TCC AGT GG -3’ Sigma-Aldrich N/A FAM-labeled RNA 5’- UUU UUU GGC AUA GUU -3’ Sigma-Aldrich N/A Primers for NELF A mutants Sigma-Aldrich N/A (Continued on next page) Molecular Cell 84, 1243–1256.e1–e5, April 4, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms CryoSPARC v4.2.0 Punjani et al.75 https://cryosparc.com WARP (v1.0.9) Tegunov et al.76 http://www.warpem.com/warp/ COOT v0.9.8.91 Emsley et al.77 https://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ PyMOL v2.5.5 Schrodinger, 2015 https://pymol.org/2/ UCSF ChimeraX v1.6.1 Goddard et al.78 https://www.cgl.ucsf.edu/chimerax/download.html Other UnoQ Bio-Rad 7200001 HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography column Cytiva 17116701 HisTrap HP His Tag protein purification column Cytiva 17524801 HiTrap Q HP anion exchange chromatography column Cytiva 17115401 HiLoad Superdex 16/600 200 pg preparative size exclusion chromatography column Cytiva 28989335 HiLoad Superdex 16/600 75 pg preparative size exclusion chromatography column Cytiva 28989333 Superose 6 Increase 3.2/300 analytical size exclusion chromatography column Cytiva 29091598 Quantifoil R 2/1; Copper 200 Mesh Quantifoil Q2100CR1 ll OPEN ACCESS Article

    Recombinant:

    Article Title: Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies 8WG16 Millipore-Sigma Cat# 05-952-I; RRID: AB_492629 Bacterial and virus strains Escherichia coli DH5-alpha New England Biolabs C2987 Escherichia coli BL21 (DE3) RIL Merck 230280 Escherichia coli DH10EMBacY Geneva Biotech N/A Biological samples Porcine thymus tissue Pel-Freez Biologicals 59451-2 Chemicals, peptides, and recombinant proteins RNA Polymerase II Vos et al., 201840 N/A SPT4 (DSIF) Bernecky et al., 201762 N/A SPT5 (DSIF) Bernecky et al., 201762 N/A NELF-A (NELF) Vos et al., 201654 N/A NELF-B (NELF) Vos et al., 201654 N/A NELF-D (NELF) Vos et al., 201654 N/A NELF-E (NELF) Vos et al., 201654 N/A NELF-A residues 1-276 (NELF) This paper N/A NELF-A R252D, V254R, L257K, D258R (NELF) This paper N/A NELF-A K272D, R273E, R274E, R275E, K276D This paper N/A TFIIS Vos et al., 201840 N/A TFIIS (DE-AA) Vos et al., 201840 N/A Deposited data Pol II-DSIF-NELF paused, post-translocated (Map A) This paper PDB: 8UHD EMD-42270 Pol II-DSIF-NELF paused, tilted (Map B) This paper PDB: 8UHA EMD-42267 Pol II-DSIF-NELF paused, post-translocated (Map C) (map only) This paper EMD-42304 Pol II-DSIF-NELF poised, pre-translocated (Map D) This paper PDB: 8UI0 EMD-42285 Pol II-DSIF-NELF poised, post-translocated (Map D) This paper PDB: 8UBG EMD-42280 Pol II-DSIF-NELF-TFIIS, poised (Map E) This paper PDB: 8UIS EMD-42303 Experimental models: Cell lines Sf9 Expression Systems 94-001 Sf21 Expression Systems 94-003 T. ni (Hi5) Expression Systems 94-002 Oligonucleotides Template 5’- CCA CTG GAA GAT CTG AAT TTA CGG GCG CAA CTA TGC CGG ACG TAC TGA CC -3’ Sigma-Aldrich N/A Non-template 5’- GGT CAG TAC GTC CGG CAT AGT TGC GCC CGT AAA TTC AGA TCT TCC AGT GG -3’ Sigma-Aldrich N/A FAM-labeled RNA 5’- UUU UUU GGC AUA GUU -3’ Sigma-Aldrich N/A Primers for NELF A mutants Sigma-Aldrich N/A (Continued on next page) Molecular Cell 84, 1243–1256.e1–e5, April 4, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms CryoSPARC v4.2.0 Punjani et al.75 https://cryosparc.com WARP (v1.0.9) Tegunov et al.76 http://www.warpem.com/warp/ COOT v0.9.8.91 Emsley et al.77 https://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ PyMOL v2.5.5 Schrodinger, 2015 https://pymol.org/2/ UCSF ChimeraX v1.6.1 Goddard et al.78 https://www.cgl.ucsf.edu/chimerax/download.html Other UnoQ Bio-Rad 7200001 HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography column Cytiva 17116701 HisTrap HP His Tag protein purification column Cytiva 17524801 HiTrap Q HP anion exchange chromatography column Cytiva 17115401 HiLoad Superdex 16/600 200 pg preparative size exclusion chromatography column Cytiva 28989335 HiLoad Superdex 16/600 75 pg preparative size exclusion chromatography column Cytiva 28989333 Superose 6 Increase 3.2/300 analytical size exclusion chromatography column Cytiva 29091598 Quantifoil R 2/1; Copper 200 Mesh Quantifoil Q2100CR1 ll OPEN ACCESS Article



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    sf21 expression systems 94 003 t ni  (Expression Systems Inc)
    99
    Expression Systems Inc sf21 expression systems 94 003 t ni
    Sf21 Expression Systems 94 003 T Ni, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sf21+expression+systems+94+003+t+ni/Sf9+Cells/pm38401543-233-212-213
    Average 99 stars, based on 1 article reviews
    sf21 expression systems 94 003 t ni - by Bioz Stars, 2026-10
    99/100 stars
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